2- Endocrine disruption in traumatic brain injury and mitochondria-targeted protective mechanisms.

 

Traumatic brain injury (TBI) patients are affected by a severe endocrine disruption, characterized by altered levels of hormones in the blood and brain. We aim to further study how these changes impact over TBI pathological mechanisms. To simulate the disease we use two models:

 

An in vitro model where we induce a mechanical damage to cells, alongside with a metabolic dysfunction, that is depriving cells of nutrients. The mechanical damage as a product of trauma leads to the massive death of neurons and glial cells, this is followed by a metabolic injury, a consequence of the destruction of cerebral vessels and arteries, thus hindering the normal flow of glucose and nutrients to the surrounding tissues. Both processes are hallmarks of traumatic brain injury. Our team has been the first group to develop this new in vitro model and it is the main one we use in our studies (Torrente et al., 2014; Baez et al., 2019a, 2019b, 2018a, 2018b)

 

In vitro traumatic brain injury model.

 

We use a penetrating brain injury to model brain trauma in animals. In this model, a needle is introduced into the murine brain and moved anteroposterior end extension, affecting both the hippocampus and the cerebral cortex, therefore impairing motor, learning and cognitive behaviour.

 

In vivo traumatic brain injury model.